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About Analisis Ekspresi Gen Penyandi Metallothionein Tipe Ii Pada Melastoma Affine L. Yang Mendapat Cekaman PH Rendah Dan Aluminium
This document summarizes a thesis that analyzed the expression of the metallothionein type II gene (MaMt2) in Melastoma affine L. under low pH and aluminum stress conditions. M. affine was exposed to different pH levels (6 and 4) and aluminum concentrations (0, 0.8, and 3.2 mM) in liquid culture for one week. Total RNA was isolated from roots and leaves separately and used to synthesize cDNA. Real-time PCR was performed to analyze MaMt2 expression levels normalized to β-actin. Low pH did not affect MaMt2 expression in leaves or roots. High aluminum concentration (3.2 mM) induced MaMt2 expression in roots but not leaves.
Detailed Information
| Author: | ['dwika a'] |
|---|---|
| Publication Year: | 2015 |
| Pages: | 45 |
| Language: | English |
| Format: | |
| Price: | FREE |
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