nuree najat faruquee

89 Pages·2017·10.46 MB·English

ISOI"'l\TI()N, PIJRIFICATION AND IMMlfNO
HIOCHtJMICAL CHARACTERIZA'rION OF
IMMITN()(.L()HULIN G ()F.JAMUNAPARI (.()AT
( IlbetallS)
C~/JJlen
)f. lJ1iesis
Su6mittetf to tlie
of
'West {}3etlfJaf Vniversity )f.nimaf anti Pis!iery Sciences
of of
in partia{j ufJiifment tli£ requirement for tli£ degree
of
Master o/eterinary Science
In
VETERINARY BIOCHEMISTRY
By
NUREE NAJAT FARUQUEE
B.V.Sc. & A.H.
DEPARTMENT OF VETERINARY BIOCHEMIS1RY
FACULTY OF VETERINARY AND ANIMAL SCIENCES
WEST BENGAL UNIVERSITY OF ANIMAL AND FISHERY SCIENCES
37 &' 68 K. B. SARANI, BELGACliIA, KOLKATA-700037.
WEST BENGAL. INDIA
2013

DEPARTMENT OF VETERINARY BIOCHEMISTRY
WEST BENGAllJNMRSITY OF ANIMAl AND FISHERY S£IEN£ES
FACULTY OF VETERINARY AND ANIMAL SCIENCES
37 & 68 KSHUDIRAM BOSE SARANI, KOLKATA-700 037
WEST BENGAL
"//1;4
13>
Dr. S. Batabyal. No: •••
M.V.Sc (IVRI), PhD (IVRI)
Head, Date:·.r"·I·9;!·~
Department of Veterinary Biochemistry
(]ElR!J1PIC }lfJ!E
This is to certify that the thesis entitled "Isolation, Purification
and Immuno-biochemical Characterization of Immunoglobulin G of
Jamunapari Goat (Capra hircus)" submitted by Dr. Nuree Najat
Faruquee, in partial fulfillment of the requirements for the 'Degree of
Master of Veterinary Science in Veterinary Biochemistry' of the West
Bengal University of Animal and Fishery Sciences, is the faithful and
bonafide research work carried out under my personal supervision and
guidance. The results of the investigation reported in the thesis have not
so far been submitted for any other Degree or Diploma.
The assistance and help received during the course of
investigation have been duly acknowledged.
Jodi ~ '~/3
f)alpd;-
Kolkata
(Dr. S. Batabyal)
Chainnan, Advisory Committee
APPROV AL OF EXAMINERS FOR THE AWARD OF THE
DEGREE OF MASTER OF VETERINARY SCIENCE IN
VETERINARY BIOCHEMISTRY
We, the undersigned, having been satisfied with the
performance of Nuree Najat Faruquee, in the Viva-Voce
the.!l:$M.a~
Examination, conducted today, 2013,
recommend that the thesis be accepted for the award of the
Degree of Master of Veterinary Science in Veterinary
Biochemistry.
NAME SIGNATURE
1. Dr. S. Batabyal Chairman,
M.V.Se., Ph.D. Advisory Committee
to :-:.~.,
3. Dr. S.Chattopadhyay Member,
M.V.Se., Ph.D. Advisory Committee ~ ......
4. Dr. P.K. Das Member, fr>
M.V.Se., Ph.D. Advisory Committee \
5. Dr. Kunal Batabyal Member,
M.V.Se., Ph.D. Advisory Committee
ACKNOWLEDGEMENT
In tliis auspicious Gut (onn ana turilulent journey I
WO?-L{c[ [ike to convey my tlianks to a[[ tliat liave contribute a to
my work, liefpea ana supportea me auring my time workinn on
tliis tliesis.
I appraise my d"eey sense of gratitude ana inaeGted"ness
to my cogent, perspicacious ana reverend major atfvisor of tlie
present stuay, Dr. S. 'BataGya[, J-{eaa & 'Reader, Department
of 'Veterinary 'Biocliemistry, :facu[ty ofYeterinary ana 5\nima(
Sciences, . West 'Be11f:Ja( 'University of ytnima[ ana :fisliery
Sciences, 68, Xsliudiram 'Bose Sarani, Xo{kata - 700037, for
unequivoca{ motivation, benevo[ent gUiaance, affectionate
encouragement, va[ua6[e cfiscussion ana constant supervision
cfuri11fJ tlie entire periocfo f investigations.
I am extreme{y grateful to the mEmber of tlie acfvisory
committee, Dr. S. Cliattopatfliyay ,'Reatfe"Y , Department of
'Veterinary 'Biochemistry, :f/O-Y5\S, W'B'U.Jl:fS, Xorkata-
700037, West 'Benga{ for liis benevol£nt guiaance, va{uaGfe
acfvice, constructive criticism, avera[ attituae ana whore
fieartea encouragement.
Wont's fairs to express my c£eep sense of gratituc£e ana
sincere tfianks to tlie mEm6er of tfie acfvisory committee, 'Dr.
Xuna[ 'Bata6ya{, 'Department of 'Veterinary Jv1.icrovio[ony for
his affectionate encouragement ana constant supervision
auring the entire perioao f investinations.
Acknowledgement
I'm afso grateful to 'Dr. P.X. 'Das, 'Reader, 'Department
of 'Veterinary PFiysio{ogy for fiis proper guidance, va{ua6fe
sU[Jgestions arui Co-operation tfirougFiout the study.
c.s.
I fee{p {easure to exterui my sincere thanks to Prof
Chakraborty, 3fon'6{e 'Vice-Chance{{or; Prof ('Dr.) 'i. x. :Monda(,
'Dean, :facu{ty of 'Veterinary and Anima{ Sciences; Prof ('Dr.)
S. 1Jiswas, Contro[{er of 'Examinations, West 'Benga[ l1niversity
of Anima{ & :fishery Sciences for providing me tFie faci{ities for
carrying out my research work.
:No appropriate words couUf 6e tracea in tfie present{y
avai{a6{e {exicon to express my deepest gratitude aruf
inde6tness to 'Dr. Sftyamsunaar Xesft, .Jtssistant Professor
('Vety. 1Jiocnemistry), 'Deptt. of '.TYee
I Jee[ great p{easure to convey my thanks to my seniors
3farka da, Sanju eli, Souvik da ,'De6jani di, :Aryana cfi, :A6fiijit
da ,1Jat{a da ,Prasun da, Cfianaana cfi.
It eives me immense joy and satisfaction wfien I reco«ect
tfie fiery aruf contribution of my co{{eaeues andf riends Sourav,
Partfia, 'Rahamat, Sonaa, 'Piyusfi, Saikat, 'Piya{i, Joytfeey,
Tarani, Nirmalya, 'Bit Iii, :Nazia,
I aCso express my sincere tfianlis to my juniors Xeya,
'Ratan, Swarnava, Saba for tfiei:r co-operation and se(f{ess
suyport.
era,
I'm thankful to 'Diyak d'a , 'Biswanatft Xur6an d'a,
era,
Tanmoy d"a, Soumya aa, .Jtyurba Tayan cfa and a[[ otfter
staff members for tlieirkimf co-oyeration a[[ a[ong tlie course of
tfie present stuay.
Acknowledgement
I am rea[[y grateful ana would Cike to express my
syeciaf thanks to my he fovea parents, eWer hrotliers, eWer
sister, Jiju ana in-raws for tfieir unfai{ing support ana
encouragement wfiich have ena6fea me to reacFi this stage of
success so far.
I express my extreme rove ana gratitude to my
hushana Zia for Fiis constant ana cFieerfuI suyyort, infinite
comyassion ana encouragement ana enafess fiefp tfiroughout
tlie whofe yeriod.
J'inaf{y to any ana a{[ tFiat I might fiave missea to
mention Fiere, I have not forgotten you ana1 never wife
_-Xwr.IA __
Vatec£:- J.rlf1h __ ~LTrJ b ~iJ._-L£=.vqt.LU
£A
Xo{kata (Nuree Najat J'aruquee)
NO
(~DJlI)'rlnl I)JlR'rJ(~(JIARS
iI. INTRODUCTION 1-3
2 REVIEW OF LITERATURE 4-23
3 MATERIALS AND METHODS 24-35
4 RESULTS 36-38
5 DISCUSSION 39-42
6 SUMMARY AND CONCLUSION 43-45
7 FUTURE SCOPE OF RESEARCH 46
BIBLIOGRAPHY I-XID
APPENDIX I-IV
(,IlJll'I1 SlJn.JEer
~().
1. Purification of crude Immunoglobulin G of
Jamunapari Goat serum by gel nItration
chromatography on 8ephacryl 8-200.
1~J(JunE NO. SlID.JEC'r
1. SDS PAGE (10% gel) showing the crude JgG fraction
along with protein molecular weight marker (Range
14.3- 97.4 kDa) (Coomassie Brilliant blue stain).
2. SDS PAGE (10%) showing the purified JgG fractions
along with protein molecular weight marker (Range
14.3- 97.4 kDa) (Coomassie Brilliant blue stain).
3. Double Immunodiffusion Test showing the precipitin
line of purified Immunoglobulin G with the
hyperlmmune serum.
4. Western Blot analysis of purified JgG (OD gel filtration
chromatography) of Jamunapari Goat.